| 1 |  Chloé Dygert-Owen (USA) | Twenty20 - Sho-Air | 51:02 |
| 2 | Katie Hall (USA) | United Health Care | zt |
| 3 |  Sara Bergen (CAN) | Rally Cycling Women | zt |
| 4 | Samantha Schneider (USA) | Iscorp - Progress Software | zt |
| 5 | Jennifer Valente (USA) | Twenty20 - Sho-Air | zt |
| 6 | Lily Williams (USA) | Hagens Berman - Supermint | zt |
| 7 |  Diana Carolina Peñuela Martinez (COL) | United Health Care | 0:04 |
| 8 | Leah Thomas (USA) | United Health Care | 0:04 |
| 9 | Leigh Ann Ganzar (USA) | | 0:04 |
| 10 | Marie-Soleil Blais (CAN) | | 0:04 |
| 11 |  Rachel Langdon (GBR) | | 0:04 |
| 12 |  Jessica Mundy (AUS) | The Meteor - Intelligentsia | 0:04 |
| 13 |  Edwige Pitel (FRA) | | 0:04 |
| 14 | Shayna Powless (USA) | Twenty20 - Sho-Air | 0:09 |
| 15 | Whitney Allison-Schultz (USA) | Hagens Berman - Supermint | 0:09 |
| 16 | Emily Newsom (USA) | Tibco - Silicon Valley Bank | 0:11 |
| 17 | Elizabeth Banks (GBR) | United Health Care | 0:11 |
| 18 | Laura Jorgensen (USA) | | 0:11 |
| 19 | Kathryn Buss (USA) | Tibco - Silicon Valley Bank | 0:11 |
| 20 | Katherine Maine (CAN) | Rally Cycling Women | 0:11 |
| 21 |  Sofia Arreola Navarro (MEX) | Twenty20 - Sho-Air | 0:16 |
| 22 | Jennifer Luebke (USA) | Hagens Berman - Supermint | 0:16 |
| 23 | Carolyn Defoore (USA) | | 0:20 |
| 24 | Josie Talbot (AUS) | Iscorp - Progress Software | 0:20 |
| 25 | Peta Mullens (AUS) | Hagens Berman - Supermint | 0:22 |
| 26 | Erica Carney Allar (USA) | Rally Cycling Women | 0:24 |
| 27 | Heidi Franz (USA) | Rally Cycling Women | 0:24 |
| 28 | Starla Teddergreen (USA) | Hagens Berman - Supermint | 1:27 |
| 29 |  Caroline Baur (SUI) | Iscorp - Progress Software | 1:43 |
| 30 | Summer Moak (USA) | Rally Cycling Women | 1:47 |
| 31 | Lauren Hall (USA) | United Health Care | 1:53 |
| 32 | Jennifer Tetrick-May (USA) | Tibco - Silicon Valley Bank | 1:59 |
| 33 | Beth Ann Orton (USA) | Point S | 2:01 |
| 34 | Catherine Ouellette (CAN) | The Cyclery | 2:01 |
| 35 | Stefanie Sydlik (USA) | Qcw Breakaway - Felt | 2:38 |
| 36 | Natalie Smith (USA) | | 2:39 |
| 37 | Emma Swartz (USA) | | 3:08 |
| 38 | Bri Kovac (USA) | | 3:08 |
| 39 | Danielle Morshead (USA) | Twenty20 - Sho-Air | 3:08 |
| 40 | Daphne Karagianis (USA) | The Meteor - Intelligentsia | 3:12 |
| 41 | Kelly Madison (USA) | | 3:12 |
| 42 |  Courteney Lowe (NZL) | | 3:12 |
| 43 | Flora Yan (USA) | Papa John's - Trek | 3:15 |
| 44 | Kendall Cail Ryan (USA) | Tibco - Silicon Valley Bank | 3:25 |
| 45 | Nicole Pressprich (USA) | Point S | 3:28 |
| 46 | Harriet Owen (GBR) | The Meteor - Intelligentsia | 3:36 |
| 47 | Hannah Shell (USA) | Papa John's - Trek | 3:40 |
| 48 | Lauretta Hanson (AUS) | United Health Care | 3:40 |
| 49 |  Nicolle Bruderer Cofino (GUA) | Tibco - Silicon Valley Bank | 3:43 |
| 50 | Emily Spence (CAN) | | 5:21 |
| 51 | Esther Meisles (USA) | | 5:21 |
| 52 | Christie Tracy (USA) | | 5:21 |
| 53 | Katie Kantzes (USA) | | 5:21 |
| 54 |  Annika Teschke (GER) | Gesundshop24.de | 5:45 |
| 55 | Carlyn Jackson (USA) | | 6:13 |
| 56 | Jessie Hodges (NZL) | Iscorp - Progress Software | 6:13 |
| 57 | Emily Rodger (CAN) | | 6:13 |
| 58 | Anna Gabrielle Traxler (CAN) | | 6:13 |
| 59 | Sheri Rothe (USA) | | 6:13 |
| 60 | Emily Flynn (CAN) | The Cyclery | 6:13 |
| 61 | Yusseli Mendivil Soto (MEX) | Iscorp - Progress Software | 6:13 |
| 62 | Lori Nedescu (USA) | | 6:13 |
| 63 | Jesica Bonilla Escapite (MEX) | | 6:13 |
| 64 | Emily Jordan (USA) | | 6:13 |
| 65 | Julie Kuliecza (USA) | Hagens Berman - Supermint | 6:13 |
| 66 | Kristen Arnold (USA) | The Meteor - Intelligentsia | 6:13 |
| 67 | Debbie Milne (USA) | Papa John's - Trek | 6:13 |
| 68 | Jennah Dunham (USA) | | 7:31 |
| 69 | Samantha Bosco (USA) | | 7:31 |
| 70 | Ashley Weaver (USA) | Papa John's - Trek | 7:31 |
| 71 | Allison Schroeder (USA) | | 7:31 |
| 72 | Megan Heath (USA) | Rally Cycling Women | 7:31 |
| 73 | Grace Chappell (USA) | | 7:31 |
| DNF | Scotti Lechuga (USA) | | - |
| DNF | Deborah Paine (NZL) | Isorex - Noaaqua | - |
| DNF | Kimberley Pettit (USA) | Papa John's - Trek | - |
| DNF | Olivia Baril (CAN) | Macogep - Argon18 - Girondins - Mazda | - |
| DNF | Tabitha Sherwood (USA) | | - |
| DNS | Masha Schneider (USA) | | - |
